Journal: BMC Microbiology

Article Title: Design of genus-specific semi-nested primers for simple and accurate identification of Enterobacter strains

doi: 10.1186/s12866-025-04175-1

Figure Lengend Snippet: Core genome tree based on 81 bacterial core genes in the type strains of 29 Enterobacter species/subspecies (blue) and 60 other Enterobacteriaceae used for the comparative genomics. Filled squares show (1) Presence of the target gene hpaB , (2) Expected small PCR amplicon 1 in members of the genus, (3) Expected long PCR amplicon 2 in members of the genus

Article Snippet: The full sequences of the marker genes atpD , dnaJ , gyrB , infB , rpoB and hsp 60 as well the sequence of the hpaBC long amplicon (370 bp) were extracted from the 4,276 Enterobacter spp. genomes using BLASTN with the corresponding sequences from E. cloacae ATCC 13,047 T ( NC_014121.1 ; locustags: ECL_RS00020, ECL_RS01185, ECL_RS22690 ECL_RS25685, ECL_RS03995, ECL_RS02580).

Techniques: Amplification

Journal: BMC Microbiology

Article Title: Design of genus-specific semi-nested primers for simple and accurate identification of Enterobacter strains

doi: 10.1186/s12866-025-04175-1

Figure Lengend Snippet: Agarose gel electrophoresis of PCR amplicons: (1) Enterobacter cloacae ATCC13047 T , (2) Enterobacter cancerogenus LMG 2693 T , (3) Enterobacter asburiae DSM17506 T , (4) Enterobacter soli LF7 T , (5) Enterobacter ludwigii ECWSU1, (6) Enterobacter kobei AZ3a, (7) Enterobacter mori 19UT013, (8) Enterobacter hormaechei subsp. steigerwaltii 20TX0131, (9) Enterobacter bugandensis 20TX0138, Enterobacter roggenkampii 20CA0095, 11. Enterobacter wuhouensis 20GA0343, 12. Leclercia pneumoniae LMG 5336, 13. Leclercia pneumoniae ATCC 27,994, 14. Leclercia pneumoniae ATCC 27,993, 15. Escherichia coli SM06, 16. Kluyvera sp. 9062RM, 17. Pseudocitrobacter sp. CIP 102,343, 18. Cronobacter sakazakii ATCC29544 T , 19. Siccibacter turicensis LMG23730 T , 20. Phytobacter diazotrophicus ATCC 27,981, 21. Buttiauxella sp. LMG 5339, 22. Pantoea agglomerans ATCC 27,155 T , 23. Franconibacter pulveris LMG24057 T , 24. negative control; M: 1 Kb Plus DNA Ladder (Invitrogen)

Article Snippet: The full sequences of the marker genes atpD , dnaJ , gyrB , infB , rpoB and hsp 60 as well the sequence of the hpaBC long amplicon (370 bp) were extracted from the 4,276 Enterobacter spp. genomes using BLASTN with the corresponding sequences from E. cloacae ATCC 13,047 T ( NC_014121.1 ; locustags: ECL_RS00020, ECL_RS01185, ECL_RS22690 ECL_RS25685, ECL_RS03995, ECL_RS02580).

Techniques: Agarose Gel Electrophoresis, Negative Control

Journal: BMC Microbiology

Article Title: Design of genus-specific semi-nested primers for simple and accurate identification of Enterobacter strains

doi: 10.1186/s12866-025-04175-1

Figure Lengend Snippet: Multi-locus sequence analysis ( A ; 10,515 bp) and single gene trees of the hpaBC PCR amplicon 2 ( B ; 370 bp) from 4276 genomes representing all species of Enterobacter , including clades of currently undescribed species

Article Snippet: The full sequences of the marker genes atpD , dnaJ , gyrB , infB , rpoB and hsp 60 as well the sequence of the hpaBC long amplicon (370 bp) were extracted from the 4,276 Enterobacter spp. genomes using BLASTN with the corresponding sequences from E. cloacae ATCC 13,047 T ( NC_014121.1 ; locustags: ECL_RS00020, ECL_RS01185, ECL_RS22690 ECL_RS25685, ECL_RS03995, ECL_RS02580).

Techniques: Sequencing, Amplification